BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C, 18-24h, aerobic |
| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it into pieces using forceps; ③ Pipette 0.5mL of liquid medium into the freeze-dried vial. After complete dissolution, return it to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspensioninto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Yellow Bulge Degree: Middle Bulge Surface: Bright and Smooth Texture: Wet and Thick |
| Sharing mode | Public welfare sharing |
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