Moisture-loving Monosporus bacteria|336468 |BNCC

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Cellulomonas uda

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspension onto plates and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under specified conditions until colonies emerge.
Growth conditions	30°C; 24-48h; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grayish-white Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

1.Description

1. Name:Cellulomonasuda

2, BNCC No.:336468

3. Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Nutritious gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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