Staphylococcus aureus|195416 |BNCC

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Micrococcus luteus

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃, 18-24 ℃, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are circular in form with an entire edge and measure medium in size. They possess a convex elevation profile. The surface is moist, smooth, and notably glistening. The colonies are opaque and pigmented yellow.
Sharing mode	Public welfare sharing

Sarcina subflava

No. 195416

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, nutritious gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 28℃ for 18-24 hours

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 20h,NB bacterial fluid turbidity
colony morphology:	size: medium color: yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: middle bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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