Aspergillus flavus|356062 |BNCC

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Aspergillus flavus

Subculture procedure	① Prepare one test tube containing 5-10 mL liquid medium and two plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then transfer back to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions	37°C; 5-7 days; aerobic
Storage conditions	2-8℃
Safety level	1
morphology	Small filamentous fungus. Colony formation is distinct on YGC medium. Initial mycelium is white, dense, and vigorous, later producing yellow spores that cover the Agar plate.
Sharing mode	Public welfare sharing

Aspergillus flavus

Storage conditions: 2~8 ℃

No. 356062

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, 5-7 days, acetic acid bacteria medium (YGC): yeast extract 5.0g, glucose 50.0g, agar 15.0g (not included in liquid medium),CaCO3 12.5g （not included in liquid medium）, distilled water 1.0L. pH 6.6 - 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 5-7days strain layer is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies on YGC medium. The initial hyphae are white, dense and vigorous, and yellow spores are covered with plates.
Conclusion:	good viability, no abnormal colony morphology, qualified

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