Taiwanese Sphingomonas|186404 |BNCC

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Sphingomonas formosensis

Culture medium	BNCC352232
Description	Tryptic Soy Broth
Composition	Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K₂HPO₄:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic;
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colonies are 1-2 mm in diameter, circular with entire margins, and opaque. The colony surface is smooth, moist, glistening, and convex in the center. They appear dark yellow in color, are easily emulsifiable, and do not produce diffusible pigments. Microscopic examination reveals Gram-negative (staining red) bacilli.
Sharing mode	Public welfare sharing