Enterococcus faecalis V347 (containing pIP501 plasmid)|361522 |BNCC

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Enterococcus faecalis V347（pIP501）

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates as described above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol burner, immediately add sterile water to break the seal, then crush the vial with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, fully dissolve the bacterial powder, and then transfer approximately 200 μL of the suspension onto each of the two plates, spreading evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	2
morphology	Purity: Pure
Sharing mode	Public welfare sharing