Streptomyces coelicolor|335934 |BNCC

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Streptomyces splendens (Yan et Deng) Yan et al.

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ The plates are all cultured under the above conditions, and the strains can be used once they grow.
Growth conditions	28 ℃; 48-72h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptomyces splendens (Yan et Deng) Yan et al

2. BNCC No.:335934

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Gao's synthetic agar No. 1: KNO3 1g, soluble starch 20g,K2HPO4 0.5g, MgSO4.7H2O 0.5g,NaCl 0.5g,FeSO4 0.01g, agar 20g, water 1000ml,pH 7.2-7.4

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4. Notes:
1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization

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