Mushroom|360540 |BNCC

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Lentinus edodes

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation.
Growth conditions	25-28 ℃; 5-7 days; aerobic;
Storage conditions	-80℃
Safety level	1
morphology	Purity: Pure
Sharing mode	Public welfare sharing