Salmonella enterica subspecies enterica|186359 |BNCC

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Salmonella enterica subsp.

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare one test tube containing 5-10mL liquid medium and two plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Colonies are light yellow, circular with entire margins, and opaque. They exhibit a convex elevation, with a smooth and moist surface. The colony size is typical for the species/conditions.
Sharing mode	Public welfare sharing