Squirrel Staphylococcus|195019 |BNCC

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Staphylococcus sciuri

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 0.5-1mm Shape: Circular Edge: Neat Transparency: Opaque Color: Milky White Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Moist
Sharing mode	Public welfare sharing

Staphylococcus sciuri

1.Description

BNCC No.：195019

Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Mannitol agar (Mannitol Agar) medium composition: yeast powder 5.0g, peptone 3.0g, mannitol 25.0g, agar 15.0g, distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

2. Atmosphere：aerobic

3. Temperature：30 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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