Brucella|187961 |BNCC

BeNa Culture Collection

Brucella-BNCC
Brucella-BNCC
  • BNCC
  • Lactobacillus buchneri-BNCC
  • Lactobacillus buchneri-BNCC

Lactobacillus buchneri

Literatures(2)
  • Price: $ 186
  • number:BNCC187961
  • MPs:
    大小:2-4mm 形状:圆形 边缘:不整齐 透明度:不透明 颜色:乳白色 隆起度:中间凸起 表面:明亮光滑 质地:湿润粘稠
  • Packing:Freeze dried pellet; Bacterial suspension; Agar plate
Essential Information References Related Products
Lactobacillus buchneri
Culture medium BNCC352238
Description MRS agar medium
Composition Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions 30 ℃; 24-48 hours; microaerophilic (3% -5% O2, 10% CO2)
Subculture procedure ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions 2-8 ℃
Safety level 1
morphology 大小:2-4mm 形状:圆形 边缘:不整齐 透明度:不透明 颜色:乳白色 隆起度:中间凸起 表面:明亮光滑 质地:湿润粘稠
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