Brucella|187961 |BNCC

BeNa Culture Collection

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Brucella-BNCC
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  • Lactobacillus buchneri-BNCC
  • Lactobacillus buchneri-BNCC

Lactobacillus buchneri

Literatures(2)
  • Price： $ 186
  • number：BNCC187961
  • MPs：
    大小：2-4mm 形状：圆形 边缘：不整齐 透明度：不透明 颜色：乳白色 隆起度：中间凸起 表面：明亮光滑 质地：湿润粘稠
  • Packing：Freeze dried pellet; Bacterial suspension; Agar plate
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Lactobacillus buchneri
Culture medium BNCC352238
Description MRS agar medium
Composition Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions 30 ℃; 24-48 hours; microaerophilic (3% -5% O2, 10% CO2)
Subculture procedure ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions 2-8 ℃
Safety level 1
morphology 大小：2-4mm 形状：圆形 边缘：不整齐 透明度：不透明 颜色：乳白色 隆起度：中间凸起 表面：明亮光滑 质地：湿润粘稠
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