Brewing yeast|336023 |BNCC

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Saccharomyces cerevisiae Hansen

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	30 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Colonies are 1-2 mm in diameter, circular with entire margins, and opaque. They are creamy white, convex, and exhibit a smooth, shiny surface with a moist and easily liftable texture.
Sharing mode	Public welfare sharing

Saccharomyces cerevisiae

storage conditions: 2~8 ℃

number: 336023

product format: freeze dried, 200ul

shelf life: 24 months

biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:25-28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:
(1)Prepare 1-2 YM plates;
(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability. in 40 hours YM plate colony is obvious
colony morphology	Size: General; color: milky white; shape: round; edge: neat; wet and smooth: bright and Opacity ; Protuberance: Bulge
Conclusion:	good recovery, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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