Stem basket shaped bacteria|226199 |BNCC

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Talaromyces stipitatus

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Cultivate the Agar plate under the above cultivation conditions for 5-7 days.
Growth conditions	28 ℃, aerobic, 5-7 days
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white and dense hyphae in the early stage, producing yellow green spores and soluble pigments in light yellow color, and yellow on the back of the medium
Sharing mode	Public welfare sharing

Talaromyces stipitatus

BNCC No.: 226199

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, comprehensive PDA,5-7 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, a little vitamin B1, agar 20.0g,pH 6.0 ±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as following:

item	test result
viability	good viability, in 5-7 days starin layer is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies on the integrated PDA medium, with white hyphae at the initial stage, dense and vigorous, yellow-green spores later, soluble pigment pale yellow, and yellow back of the medium.
Conclusion	good viability, no abnormal colony morphology, qualified

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