BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃, aerobic, 5-7 days |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Cultivate the Agar plate under the above cultivation conditions for 5-7 days. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white and dense hyphae in the early stage, producing yellow green spores and soluble pigments in light yellow color, and yellow on the back of the medium |
| Sharing mode | Public welfare sharing |
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