Black and white whorl fungus|360661 |BNCC

Essential Information Certificate Related Products

Verticillium albo-atrum

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 3-5 days; Aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, irregular shape, irregular edges, opaque, light pink, yellow on the reverse side, light color, rough surface, dry texture
Sharing mode	Public welfare sharing

Verticillium albo-atrum

Storage conditions: 2~8 ℃

No. 360661

Product format; freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28°C, aerobic, potato dextrose agar, 3-5 days. Potato dextrose agar: 1.0L potato cooking liquid, 20.0g glucose, 15.0g agar, natural pH. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5 days plate colonies are obvious
colony morphology:	small filamentous fungi, with obvious colonies on potato glucose agar medium, the prophase of the plate mycelium is white, and gradually It turns pale pink, and the back of the medium is pale yellow.
conclusion: good viability, no abnormal colony morphology, qualified

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