BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation. |
| Growth conditions | 30 ℃; 5-7 days; aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Spread and grow |
| Sharing mode | Public welfare sharing |
Mucor racemus racemosus f. racemosus
Storage conditions : 2~8 ℃
No. : 336638
Product format: agar slant in 14mm test tube
Validity period : 30 days
Biosafety level : 1 , handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.
Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.
Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4)Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions,5-7 days.Or contact our technicians for the inoculation of agar slant - filamentous fungi.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

| item | test results |
| viability | good viability, in 6 days strain layer is obvious |
| colony morphology | small filamentous fungi, colonies are obviously visible on comprehensive PDA culture medium, colonies are brown, hyphae are vigorous and plush |
| conclusion | good viability, no abnormal colony morphology, completely consistent with the above figure, qualified |