Clostridium sporogenes

No. : 353760

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level :  1

Validity period: 5 years

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : liquid culture medium thioglycolate liquid culture medium (formula as follows); The solid culture medium is Columbia blood plate (ready-to-use finished product); Thioglycolate: 15.0g of caseptone (trypsin hydrolysis), 5.0g of yeast extract, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 2.5g of sodium chloride and 0.001g of azure, agar 0.75g, distilled water 1.0L,pH 7.1 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a φ18mm sterile test tube of 25ml liquid medium; Silica gel plug is used for the liquid test tube;

(2)Sterilize the ampoule, use a small grinding wheel to make a circle at 1/2 of the tube body under strict aseptic conditions,, and then break it

(3)Draw 0.5ml of liquid growth medium into a freeze dried ampoule, make it full dissolved;

(4)Transfer all the solution to the liquid test tube, plug the tube with silica gel.

(5)Place the liquid test tube in incubator under the above growth conditions for cultivation, The culture solution is obviously turbid or precipitated, and this indicates the bacterial grows well.

Growth conditions Temperature: 37 ℃ ; Gas environment: anaerobic  Time: 48-72h