BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 28-24h; aerobic |
| Subculture procedure | ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, followed by crushing with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried tube, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Pale yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted |
| Sharing mode | Public welfare sharing |
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