Pyruvate-reactive Young Titan bacillus|192111 |BNCC

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Cronobacter malonaticus

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, followed by crushing with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried tube, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	37°C; 28-24h; aerobic
Storage conditions	2-8°C
Safety level	2
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Pale yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

Description

1. Name:Taonella mepensis

2. BNCC No.: 195681

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1, Acetobacter gluconate culture medium :glucose 20.0g, yeast extract 5.0g , K2HPO4 1.0g,MgSO4 15.0g, anhydrous ethanol 5ml, distilled water 1.0L,PH:4.5.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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