Bifidobacterium animalis subsp. lactis|192317 |BNCC

Essential Information References Related Products

Bifidobacterium animalis subsp. lactis

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37°C; 48-72h; anaerobic
Subculture procedure	① Prepare two plates as described above; ② Disinfect the ampoule surface, open it in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it open with forceps; ③ Aspirate approximately 0.5 mL sterile water (previously equilibrated in anaerobic conditions for 24 hours) into the freeze-dried vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates with 200 μL each, spreading evenly; ④ Incubate plates under the specified conditions.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque; white upper surface with central convexity; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing