Bacillus glutamicis|192544 |BNCC

Essential Information Certificate Related Products

Corynebacterium glutamicum

Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture; break open with forceps; ③ Pipette 0.5 mL sterile water into freeze-dried vial, dissolve powder completely, then dispense 200 μL onto each of 2 plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	30°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1–2 mm; circular with neat margins; opaque; yellow upper surface; centrally raised; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Corynebacterium glutamicum

2. BNCC No.:192544

3. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Glutamic acid medium: peptone 10.0g, beef paste 10.0g, sodium chloride 5.0g, glucose 1.0g, agar 20g, distilled water 1L,pH6.8-7.0,121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate