BeNa Culture Collection
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| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Growth conditions | 37°C; 18-24h; aerobic |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 4-6mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Bright and smooth Texture: Moist and easily lifted |
| Sharing mode | Public welfare sharing |
Bacillus sphaericus
No.: 193424
Storage conditions: 2~8 ℃
Product format： freeze dried, 200ul
Validity period: Freeze-dried tube for 24 months
Biosafety level: 1, handle in ultra-clean table or biosafety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:37 ℃, aerobic, nutritious agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.
Recovery steps:
(1)Prepare a flask of NB liquid media or two NB agar plates.
(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test results |
| Viability: | good viability,in 20h NB bacterial fluid is turbid, NA plate colony is obvious |
| colony morphology: |
size: larger color: orange-white shape: round edge: neat edge wet and dry: slightly dry and smooth: dark transparency: opaque uplift: flat, low convex |
| conclusion: |
good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified |
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