Bacillus circulans|194599 |BNCC

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Bacillus circulans

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then dispense 200 μL onto each of 2 plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1–2 mm; circular, with neat, opaque edges; grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing