Bacillus sphaerospermos|194789 |BNCC

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Sphingobacterium sp.

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried tube, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	30°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grayish-white Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

Sphingobacterium sp.

No. 194789

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul or flat plate 1

Validity period: Freeze-dried tube 24 months, flat plate 7 days

Biosafety level: 1, handle in ultra-clean table or biosafety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, Colombian blood plate (finished product).

Recovery steps:

(1)Prepare 1-2 pieces of Blood agar plates;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

(4)Put the agar plates under the above culture conditions for cultivation 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h Colombian blood plate colony is obvious
colony morphology:	size: medium color: pink orange shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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