Bacillus amyloliquefaciens|223006 |BNCC

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Bacillus amyloliquefaciens

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open the tube safelyopen in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 2-4 mm; circular with wavy edges; opaque; grayish-white upper surface; flat morphology; rough texture; dry consistency; easily lifted; Gram-positive (blue-violet); spore production clearly visible under microscopy after 24-hour incubation; bacilli; Purity: Pure
Sharing mode	Public welfare sharing