Locust root nodule fungus|358900 |BNCC

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Rhizobium sophoriradicis

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Transparent
Sharing mode	Public welfare sharing

Rhizobium sophoriradicis

Storage conditions : 2~8 ℃

No. : 358900

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, nitrogen fixing medium, 24-48h. Nitrogen fixation medium: KH2PO4 0.2g,K2HPO4 0.8g,MgSO4 ·7H2O 0.2g,CaSO4 ·2H2O 0.1g,FeCl3 trace g,Na2MoO4 ·2H2O trace g, yeast extract 0.5g, mannitol 20.0g, agar 20.0g, distilled water 1.0L,pH 7.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare the above flat plate 1-2 block;

② After disinfecting the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of sterile water and put it into the freeze-drying tube. after fully dissolving the bacterial powder, put it into the plate 200μL/piece, coated evenly;

④ Place the plate under the above culture conditions for 24-48h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability in 24-48 hours,strain layer is obvious;colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Transparent Color: Milk White Protuberance: Middle convex surface: bright and smooth texture: moist and viscous
conclusion: good viability, no abnormal colony morphology, qualified

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