Lipolytical Saccharomyces|225429 |BNCC

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Yarrowia lipolytica

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	30°C, 24-48h, aerobic
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it into pieces using forceps; ③ Pipette 0.5mL of liquid medium into the freeze-dried vial. After complete dissolution, transfer it back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspensioninto each Agar plate, spread evenly, and repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Milky white Elevation: Flat Surface: Rough and wrinkled Texture: Dry
Sharing mode	Public welfare sharing