Bacillus amyloliquefaciens subsp. amyloliquefaciens|191871 |BNCC

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Bacillus amyloliquefaciens subsp.amyloliquefaciens

Culture medium	BNCC360815
Description	Nitrogen fixing medium
Composition	KH₂PO₄:0.2g, K₂HPO₄:0.8g, MgSO₄.7H₂O:0.2g, CaSO₄.2H₂O:0.1g, FeCl₃:Trace, Na₂MoO₄.2H₂O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 2-4mm Shape: Circular Margins: Irregular Transparency: Opaque Color: Pale yellow Elevation: Flat Surface: Rough and dull Texture: Dry
Sharing mode	Public welfare sharing