Aspergillus niger|336168 |BNCC

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Aspergillus niger van Tieghem

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The bacteria have obvious colonies on the comprehensive PDA medium. The initial colonies are light yellow, dense and vigorous, and later produce brownish yellow spores that spread and grow towards the edge of the Agar plate, with folds
Sharing mode	Public welfare sharing

Aspergillus niger van Tieghem

Storage conditions: 2~8 ℃

BNCC No.：336168

Product format： freeze dried, 200ul

Validity period: 24 months

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:28-30 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 2 pieces of PDA plates or 1-2 agar slants;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; or transfer appropriate solution to
the agar slant.

(4)Place the plates uprightly and agar slant obliquely under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 7 days strain layer is obvious
colony morphology	The morphology of the bacteria is obviously visible in the integrated PDA agar medium. The colony is light yellow at the beginning, and brown-yellow spores are produced later. They are dry and vigorous, and spread radially on the surface of the plate.
conclusion	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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