Streptomyces|336786 |BNCC

BeNa Culture Collection

Streptomyces-BNCC
Streptomyces-BNCC
  • BNCC
  • Streptomyces sp.-BNCC
  • Streptomyces sp.-BNCC

Streptomyces sp.

  • Price: Contact
  • number:336786
  • Form:
    Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque
Standard strain Quantitative strain DNA extraction
Package:
Essential Information Certificate Related Products
Streptomyces sp.
Subculture procedure ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions 30 ℃; 24-72 hours; aerobic
Storage conditions 2-8 ℃
Safety level 1
morphology Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque
Sharing mode Public welfare sharing

Streptomyces albulus

Storage conditions : 2~8 ℃

No. : 336786

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :28-30 ℃, aerobic, Gao's synthesis medium No. 1, 24-72h. Gao's synthesis medium No. 1: soluble starch 20g, sodium chloride 0.5g, ferrous sulfate 0.01g, potassium nitrate 1.0g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, agar 15g, distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned agar plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                          

item test results
viability good viability, in 24-72h liquid medium become turbid,  obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology: (above) size: 1-2mm shape: round edge: neat transparency: opaque color: white uplift: flat surface: rough texture: dry and firm
conclusion good viability, no abnormal colony morphology, qualified
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