Streptomyces rapamycin|337576 |BNCC

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Streptomyces rapamycinicus

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Filamentous fungi have obvious colonies on Streptomyces medium No. 2, with white, dense, and flat hyphae that vigorously spread and grow
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces hygroscopicus subsp. hygroscopicus

2. BNCC No.:337576

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. ISP-2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH7.2±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3, Temperature: 26 ℃

4. precautions

1. Routine culture time: bacteria 24-48h, yeast 72h, mold 5-7d, macrofungi 7-10d.

2. Please transfer the inclined plane of the test tube as soon as possible. the storage time shall not exceed 3 months.

3. For the first time, please activate according to the recommended conditions in this manual. BNCC is not responsible for the loss of inactivation of strains caused by other types of culture media or culture conditions.

4. Bacteria-carrying waste should be discarded after autoclaving.

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