Loge Yeast|336101 |BNCC

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Saccharomyces logos van laer et Denamur ex Jorgensen

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28°C; 24-48h; aerobic;
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, cauterize top with alcohol lamp, then quickly add sterile water to rupture; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried tube, dissolve completely, then return to liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Milky white Elevation: Central dome Surface: Bright and smooth Texture: Moist and viscous
Sharing mode	Public welfare sharing