Oriental Isa Yeast|337316 |BNCC

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Issatchenkia orientalis

Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Break open using forceps; ③ Pipette 0.5mL sterile water into freeze-dried tube. Thoroughly dissolve powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	37°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat, opaque edges; pale yellow upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G- (red); bacillus; purity: pure
Sharing mode	Public welfare sharing

1.Description

1.Name: Issatchenkia orientalis

2.BNCC No.: 337316

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3.Growth Conditions
1. Name of culture medium: YM culture medium
2. Medium components: peptone 5.0g, glucose 10.0g, yeast powder 3.0g, malt extract 3g, distilled water 1.0L, agar 20.0g,pH6.2.
3. Atmosphere: aerobic
4. Temperature: 26-28 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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