BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Break open using forceps; ③ Pipette 0.5mL sterile water into freeze-dried tube. Thoroughly dissolve powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, with neat, opaque edges; pale yellow upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G- (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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