Bacillus lysaeucus|337428 |BNCC

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Lysinibacillus sphaericus

Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	30°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on top, flat in shape, smooth surface, glossy appearance, moist texture, easily liftable; G+ (blue-violet), bacillus; Purity: Pure
Sharing mode	Public welfare sharing

1.Description

1.Name: Lysinibacillus sphaericus

2.BNCC No.: 337428

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Nutritious gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2Owhen culturing Bacillus,which is beneficial to spore production.

2.Atmosphere: aerobic

3.Temperature: 30 ℃,24h

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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