Salmonella Enteritidis subsp. Enteritidis Typhimurium Serotype|351988 |BNCC

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Salmonella enterica subsp. enterica serovar Typhimurium

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, bright on the surface, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Clostridium novyi

Storage conditions: 2~8 ℃

No. 351988

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),2-3 days.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates(put in an anaerobic environment for deoxygenation in advance for 24 hours);
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water(put in an anaerobic environment for deoxygenation in advance for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3 days strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opacity Color: off-white uplift: middle convex surface: gray and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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