Salmonella Enteritidis subsp. Enteritidis|315895 |BNCC

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Salmonella enterica subsp. enterica

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24 hours; Aerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 2-4mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Grey White Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Wet and easy to pick up
Sharing mode	Public welfare sharing