Chiayi Lactobacillus|379976 |BNCC

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Chiayi Lactobacillus-BNCC
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  • Lacticaseibacillus chiayiensis-BNCC

Lacticaseibacillus chiayiensis

  • Price： $ 223
  • number：BNCC379976
  • MPs：
    The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis
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Lacticaseibacillus chiayiensis
Culture medium BNCC352238
Description MRS agar medium
Composition Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions 37 ℃; 24-48 hours; anaerobic;
Subculture procedure Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions 2-8 ℃
Safety level 1
morphology The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis
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