Chitosan-degrading Bacillus|132572 |BNCC

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Paenibacillus chitinolyticus

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18–24 h; aerobic;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to crack it open, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once growth appears.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 1–2 mm in diameter, round, with irregular edges; they are opaque, grayish-white on the upper surface, and have a smooth surface; bacilli; purity: pure
Sharing mode	Public welfare sharing