BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 24–48 h; aerobic; |
| Subculture procedure | ① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to crack it open, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once colonies appear. |
| Storage conditions | 2–8°C |
| Safety level | 1 |
| morphology | Colonies are 0.5–1 mm in diameter, round, with smooth, well-defined edges; they are opaque, white on the upper surface, convex in the center, with a smooth, lustrous surface; they have a moist texture, are easily lifted, are G+ (blue-violet), and are cocci; purity: pure |
| Sharing mode | Public welfare sharing |
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