BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 24–48 h; Anaerobic |
| Subculture procedure | ① Prepare 1–2 of the above agar plates (place in an anaerobic environment 24 hours in advance to remove oxygen); ② Open in a safety cabinet, scorch the top with a酒精 lamp, then quickly add a drop of sterile water to crack the surface, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water (previously placed in an anaerobic environment for 24 hours to remove oxygen) into the freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the aforementioned conditions; the culture is ready for use once colonies appear. |
| Storage conditions | 2–8°C |
| Safety level | 2 |
| morphology | Colonies are 1–2 mm in diameter, circular, with smooth, well-defined edges; they are opaque, white on the upper surface, convex in the center, with a smooth, lustrous surface; they have a moist texture, are easily lifted, are G+ (blue-violet), oval in shape, and have a purity rating of “pure.” |
| Sharing mode | Public welfare sharing |
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