Human intestinal Christensen bacteria|361276 |BNCC

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Christensenella intestinihominis AF73-05CM02

Culture medium	BNCC373003
Description	PY Medium with X (under N₂/CO₂ Atmosphere)
Composition	Trypticase Peptone:5.0g, Peptic Digest of Meat:5.0g, Yeast Extract:10.0g, Salt Solution(Medium 104):40.0ml, Sodium Resazurin:0.0005g, L-Cysteine.HCl.H₂O(C₃H₇NO₂S.HCl.H₂O):0.5g, Na₂CO₃:1.0g, D-Glucose:5.0g
Growth conditions	37 ℃; 3-4 days; anaerobic;
Subculture procedure	① Dissolve the Frozen vial tube in a water bath (bacteria 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into a test tube (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing