BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 28°C; 24–48 h; aerobic; |
| Subculture procedure | ① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to rupture it, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial, dissolve the bacterial powder thoroughly, then pipette approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once colonies appear. |
| Storage conditions | 2–8°C |
| Safety level | 1 |
| morphology | Colonies are 0.5–1 mm in diameter, circular with smooth, well-defined edges; the surface is orange, with a raised center, smooth texture, and moist consistency; G+ (blue-violet), rod-shaped |
| Sharing mode | Public welfare sharing |
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