Klebsiella pneumoniae subsp. pneumoniae|186055 |BNCC

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Klebsiella pneumoniae subsp. pneumoniae

Culture medium	BNCC373763
Description	Nutrient Agar with 1% Glucose
Composition	Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, Glucose:10.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	26°C; 18–24 h; aerobic;
Subculture procedure	① Prepare 1–2 plates as described above; ② Open in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to rupture it, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial, dissolve the bacterial powder thoroughly, then dispense approximately 200 μL onto each of the two plates, spreading evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once growth appears.
Storage conditions	2–8°C
Safety level	2
morphology	Colony diameter: 1–2 mm; circular; with smooth, well-defined edges; opaque; yellow on the surface; centrally elevated; smooth surface; moist texture; does not produce pigment; G– (red); bacillus; Purity: Pure
Sharing mode	Public welfare sharing