Shigella sonnei|108852 |BNCC

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Shigella sonnei

Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. Thoroughly dissolve powder, then inoculate two plates (200μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	37°C; 18-24h; Aerobic
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular with neat margins; opaque; pale yellow upper surface; central elevation; smooth, moist texture; easily liftable; Gram-negative (red); short rod-shaped
Sharing mode	Public welfare sharing

Shigella sonnei

Storage conditions: 2~8 ℃

No.: 108852

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level : 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37°C, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation,and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 18h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle raised surface: bright and smooth texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

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