Klebsiella pneumoniae|102997 |BNCC

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Klebsiella pneumoniae

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18–24 h, aerobic
Subculture procedure	① Prepare 1–2 plates as described above; ② Open in a safety cabinet; following the arrow markings on the vial cap, open the strain and reconstitution solution; ③ Add 1 mL of reconstitution solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Pipette 200 μL of the bacterial suspension into each of the two plates and spread evenly; ⑤ Incubate the plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2–8°C
Safety level	2
morphology	Colony diameter: 1–2 mm; circular; well-defined edges; opaque; pale yellow on the surface; centrally elevated; smooth surface; moist texture; easily lifted; G– (red); bacillus
Sharing mode	Public welfare sharing