BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C, 18–24 h, aerobic |
| Subculture procedure | ① Prepare 1–2 plates as described above; ② Open in a safety cabinet; following the arrow markings on the vial cap, open the strain and reconstitution solution; ③ Add 1 mL of reconstitution solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Pipette 200 μL of the bacterial suspension into each of the two plates and spread evenly; ⑤ Incubate the plates under the specified conditions; the strain is ready for use once growth appears. |
| Storage conditions | 2–8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1–2 mm; circular; well-defined edges; opaque; pale yellow on the surface; centrally elevated; smooth surface; moist texture; easily lifted; G– (red); bacillus |
| Sharing mode | Public welfare sharing |
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