Streptococcus pyogenes A|102663 |BNCC

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Streptococcus hemolytis-α

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; 5% CO2
Storage conditions	room temperature
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Streptococcus hemolytis-α

No.: 102663

Product format :freeze dried,200ul

Storage conditions: 2~8 ℃

Validity period: Freeze-dried tube for 6 years

Biosafety level: 2, handle in biosafety cabinet

notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37 ℃, aerobic/5% CO2, Columbia blood plate (finished product).

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

project	test results
viability	good viability,in 24 hours Colombian blood plate colonies are obvious
colony morphology:	size: general color: off-white shape: round edge: neat edge wet and dry: moist and smooth: smooth transparency: translucent, hemolytic uplift: flat
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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