Enterococcus faecalis|102668 |BNCC

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Enterococcus faecalis

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 18–24 h; aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2–8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular shape with neat margins; opaque; white upper surface, grayish-white underside; centrally raised; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); cocci
Sharing mode	Public welfare sharing