Aspergillus oryzae|187694 |BNCC

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Aspergillus oryzae

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, producing green spores with a darker color and a golden yellow color on the reverse side
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 187694

Product format: freeze dried, 200ul

Validity: 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Gorwth conditions: 28 ℃, aerobic, wort medium, 3-5 days. Malt juice medium: malt extract 20.0g, glucose 20.0g, peptone 1g, agar 20.0g, pH natural. Sterilization at 121 ℃ for 15min.

Receiving notice: If any abnormality is found on the day of receipt, please contact customer service in time within 24 hours. The tube bevel can be used directly. In principle, it can be used many times without stain during the storage period, but the vitality will gradually decrease with time. Please operate in strict accordance with the instructions. Otherwise, the replacement service will not be provided if abnormal strains and inactivation are caused.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put all the plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5days strain layer become obvious
colony morphology:	small filamentous fungi with obvious colonies on wort medium, White, dense and vigorous hyphae, green spores later, spreading to the edge of the plate, and golden yellow on the back of the medium.
Conclusion:	good viability, no abnormal colony morphology, qualified

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