Aspergillus fumigatus|238133 |BNCC

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Aspergillus fumigatus Fresenius, anamorph

Subculture procedure	① Prepare 1-2 agar plates as described above.② Sterilize the surface of the ampoule vial. Open it within a biological safety cabinet. Briefly flame the top, then immediately apply a drop of sterile water to crack it. Use sterile forceps to break it open.③ Aspirate 0.5 mL of sterile water and inject it into the freeze-dried vial. Mix thoroughly to completely resuspend the bacterial powder. Transfer 200 μL of the suspension onto each agar plate and spread evenly.④ Incubate the plates under the aforementioned culture conditions for 5-7 days.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The fungus exhibited white, floccose mycelium at the early stage, developing into a dark smoke-green colony with a wrinkled reverse on the agar medium at the later stage.
Sharing mode	Public welfare sharing

Aspergillus fumigatus

Storage conditions: 2~8 ℃

No. 238133

Product format： freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, comprehensive PDA,5-7 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5-7 days plate colony is obvious
colony morphology: (above)	small filamentous fungi have obvious colonies on the integrated PDA medium, with white hyphae at the initial stage, dense and vigorous, and gray spores growing on the plate later.
Conclusion	good viability， no abnormal colony morphology, qualified

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