Clostridium sporogenes|336899 |BNCC

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Clostridium sporogenes

Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take about 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 24-48 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Colonies are 0.5-1 mm in diameter, circular, opaque, with irregular margins.
Sharing mode	Public welfare sharing

1.Description

1. Name: Clostridium sporogenes

2. BNCC No.:336899

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Thioglycolate medium: caseptone 15.0g, yeast leaching powder 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, azure 0.001g, sodium chloride 2.5g,pH7.1±0.2. 121 ℃,15min.

2. Atmosphere: anaerobic

3. Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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