Bacillus subtilis|109047 |BNCC

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Bacillus subtilis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 18-24h; Aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μ L of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The diameter of the colony is 2-4mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough on the surface, dry texture, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing