Fusarium graminearum|113713 |BNCC

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Fusarium graminearum

Subculture procedure	① Dissolve the cryovial in a water bath (mold at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap and transfer the solution and its bacterial block into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; Aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus, forming white mycelium on the obverse side of the medium with spreading growth, rough surface, and dry texture. The reverse side of the medium appears yellow.
Sharing mode	Public welfare sharing

Gibberella zeae (Schwein.) Petch

Storage conditions : 2~8 ℃

No. : 113713

Product format : agar slant in 14mm test tube

Validity period: 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growthconditions :25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 5 days plate colony is obvious
colony morphology:	small filamentous fungi, with obvious colonies on comprehensive PDA medium, the front hyphae are cotton wool-like, light magenta, dense and vigorous, No exudate; yellow on the back;
Conclusion:	good viability, no abnormal colony morphology, qualified

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